Mouse Fibroblast L929 Cell Line as a Useful Tool for Replication and Adaptation of Infectious Bursal Disease Virus.

Infectious bursal disease virus (IBDV) causes a highly contagious disease associated with immunosuppression in young chickens. Production of either egg-based or primary cell-based high-quality vaccines requires time-consuming and costly procedures. To determine a suitable cell line for IBDV replication, L929 cell line was a candidate for the growth kinetics processing of the virus. The L929 cells were proliferated in monolayer, and doubling time was calculated. Replication kinetics an IBDV isolate at the multiplicity of infection 0.1 PFU/cell were determined using virus titration. To adapt IBDV on L929 cells, seven consecutive passages were performed. Virus titer and levels of apoptosis were quantitatively analyzed at each passage. The viral VP2 gene was amplified and sequenced in three passages. An average doubling time of 21 h was estimated for monolayers of L929 cells. Although during early passages, virus growth did not produce a clear cytopathic effect (CPE), an increase in IBDV titers was observed. Serial passages led to the evidence of marked CPEs and an increase in the virus titer in the third passage. During the fourth to seventh passages, consistent CPEs characterized by the formation of granulated and round cells were evident within 24 to 48 hours post-inoculation. The titer of the virus was increased in the third passage onwards to peak in the fourth and constant at 5.9 TCID50 until the end passage. The IBDV replication in connection with DNA fragmentation and FITC, revealed the characteristic picture of apoptosis in a time-dependent manner. We found that the IBDV could easily be adapted to L929 cells, increasing virus yields by about two orders of magnitude. These results indicated that the cell line may be useful in the production of efficient virus particles.

Novel Applications of Immuno-bioinformatics in Vaccine and Bio-product Developments at Research Institutes.

There are many challenges in the field of public health sciences. Rational decisions are required in order to treat different diseases, gain knowledge and wealth regarding research, and produce biological or synthetic products. Various advances in the basic laboratory science, computer science, and the engineering of biological production processes can help solve the occurring problems. Bioinformatics is defined as a field of science combined of biology, mathematics, physics, chemistry, and computer sciences. Recently, bioinformatics has been extensively used in the designing of the epitope, vaccines, antibodies, adjuvants, diagnostic kits, and therapeutic purposes (e.g., proteins, peptides, or small molecules). Moreover, bioinformatics includes chemoinformatics that has been employed to produce various biological or chemical products to target and combat pathogens. Bioinformatics is involved in other areas of data analysis and prediction, such as structural biology, system biology, phylogeny, population genetics, and next-generation data sequencing. To the best of our knowledge, no published study coherently described the benefits of bioinformatics fields applied for medication development or diagnostic aims in bio-productive and pharmaceutical/vaccine companies. Therefore, in the current review, we attempted to present the available bioinformatics resources, practical experiences, and other findings in the mentioned field along with providing a harmonized and applied model(s). The key points presented in the current review may help to elevate production and reduce the costs for the development of novel vaccines, medicines, and antibodies. In addition, these methods can facilitate the identification of organisms and may guarantee the quality of biological products.

Campylobacter jejuni and Campylobacter coli in cecum contents of chickens of slaughter age: A microbiological surveillance.

The presence of foodborne pathogens is a major concern for the food industry and increase in antibiotic resistance adds to the seriousness of this issue. Epidemiological studies have shown that there is little or no information from Iran on the prevalence of Campylobacter spp. in chickens of slaughter age. The aim of this study was to determine the prevalence, antibacterial susceptibility and type of Campylobacter species isolated from the cecum of chickens bred in Saqqez city, Kurdistan, western Iran. Campylobacter was isolated and identified by culture and molecular methods. Antibiotic susceptibility of Campylobacter species was performed by disk agar diffusion test and agar dilution methods. The bacterial isolates were typed by repetitive element sequence based polymerase chain reaction (rep-PCR) method. Fifty-five percent of the farms were found to be contaminated with Campylobacter spp. Gene amplification assay confirmed 67 isolates with Campylobacter spp., of which 57 (85.1%) were identified as C. jejuni and 10 (14.9%) as C. coli. Resistance to tetracycline was the most common finding (70.6%), followed by ciprofloxacin (63.7%) and amoxicillin (27.5%). All isolates retained their susceptibility towards gentamicin and meropenem. Results of MIC50 and MIC90 confirmed high resistance towards tetracycline and ciprofloxacin. Repetitive element sequence based-polymerase chain reaction (rep-PCR) placed C. jejuni in six profiles, while C. coli could not be separated as diverse clones. The present study focused on obtaining data regarding prevalence, antibiotic susceptibilities, genetic diversity at regular intervals and maintain and improve hygiene. The results of this study showed substantial genetic diversity of C. jejuni in chickens from western Iran.